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Related post: P€«ioo covEPao October 1, 1991 to September 30, 1992 TITUE OP PROJECT fSO ch^na^rx or fctt TJr*. tx^a Ot on orw fcvt £«Erectalis Tablets Clinical Investigation Mucosal Immunity Section INSTITUTE AND LOCATION NIAID, NIH, Bethesda, Maryland 20892-0010 TOTAL STAFF YEARS: l.( PROFESSIONAL- 0.5 0.5 a^eCX APPROfYOATE BOXIESl CS (a) Human subjects D (a 1 ) Minors D (a2) Interviews (b) Human tissues D (c) Neither SLfMMARy Of work; (Ut. 0n«c fr. ,u T^^ ^T J P?-''^^^ '^ ^° ^""'^^^^ ^^^'"^ ^^^ immunopathogeneic mechanisms responsible tor the clinical syndrome known as common variable immunodeficiency (CVI) Dunne the past year we extended our studies on the functional properties of the expanded population of CD8 T cells seen in a subgroup of patients with CVI. We demonstrated that these cells exhibit a defect in ?Tnd?.nr , b P'°?^'^^l^"-. However, these CD8 T cells showed increased production of IL- 5 and gamma-interferon. In addition, we found that these T cells were fully functional as cytotoxic 1 ymphocytes in anti-CD3 mediated cytotoxicity assays and exhibited increased suppressor nS^c f"'"^'"""oglobulin production. Taken together, this phenotypic and functional profile suggests that the expanded CDS Tcell subpopulation in this group of CVI patients contain standpoint but which nevertheless are immunoreactive from the point of view of gamma- IhTthe Cn« T fn r '""^ ^y^oio^^'c/suppressor activity. These findings raise the possibility !^h>. ^P\T^^«"s '" this group of CVI patients are "foot prints" of a chronic viral infection wtiich is the basic cause of the disease. In a separate line of investigation, we been able, for tlie first time, to propa" an abnortiial hne^ e.^?hl!ch H f ^^ produce reducedamounts of immunoglobulins in vitro when compared to mn Erectalis Online unnShnf °^ "^""' ^^"^^^^T^^, addition of a vanety of lymphokines failed to normalize immunoglobulin production by CVI B cell lines. Electron microscopy and reverse transcnptase assays perfomied on the patient B cell lines failed to provide evidence of viral infection Preliminary molecular studies performed on these cell lines suggest that the B cell defect in CVI may relate in part to a failure of immunoglobulin isotype switch. The ability to obtain large mXrMll "^r'"'^''"'}'^. ^XwPo '"' ''^""'^ 8'"^^"y f'-^^"''^'^ -^'^'d'^^ '"^o ^he biochemical and molecular mechanisms of the CVI B cell defect. 5-32-1 DEPARTMENT OF HEALTH AND HUMAN SERVICES - PUBLIC HEALTH SERVICE NOTICE OF INTRAMURAL RESEARCH PROJECT PROJECT NUMBER ZOl AI 00595-01 LCI PERIOD COVERED October 1, 1991 to September 30, 1992 TITLE OF PROJECT (80 characters or less. TiUe must fit on one line between the borders.) Purification, Characterization and Function of Proteins of the Complement System PRINCIPAL INVESTIGATOR (List other professional personnel bekm the Principal Investigator.) (Name, title, laboratory, and institute affiliation) PI: C. Hammer - Senior Investigator - LCI, NIAID M. Frank - Chief - LCI, Buy Erectalis NIAID T. Leto - Senior Staff Fellow - LCI, NIAID T. Russo, Senior Staff Fellow - LCI, NIAID R. Jacobs - Medical Staff Fellow - LCI, NIAID G. Kerr, Visiting Fellow - LCI, NIAID G. Linton - Medical Technologist - LCI, NIAID L. Renfer - Chemist - LCI. NIAID COOPERATING UNITS (if any) LAB/BRANCH Laboratory of Clinical Investigation SECTION Clinical Immunology Section INSTITUTE AND LOCATION NIAID, NIH, Bethesda, Maryland 20892 TOTAL STAFF YEARS: 3.0 PROFESSIONAL: 1.3 OTHER: 1.7 CHECK APPROPRIATE BOX(ES) n (a) Buy Erectalis Online Human subjects n (a1) Minors
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